Development and Application of CRISPR-Cas Based Tools
Yanping Hu, Wei Li
Abstract
Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future.
Topics & Concepts
CRISPRNucleaseComputational biologyGenome editingComputer scienceNucleic acidBiologyRNADNAGeneticsGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesViral Infections and Immunology Research