Sml1 Inhibits the DNA Repair Activity of Rev1 in Saccharomyces cerevisiae during Oxidative Stress
Rui Yao, Pei Zhou, Chengjin Wu, Li Liu, Jing Wu
Abstract
Rev1 was critical for cell growth in S. cerevisiae , and the deletion of REV1 caused a severe growth defect in cells exposed to oxidative stress (2 mM H 2 O 2 ). Furthermore, we found that Sml1 physically interacted with Rev1 and inhibited Rev1 phosphorylation, thereby inhibiting Rev1 DNA antioxidant activity. These findings indicate that Sml1 could be a novel regulator for Rev1 in response to DNA damage by oxidative stress.
Topics & Concepts
Saccharomyces cerevisiaeOxidative stressDNA repairDNADNA damageOxidative phosphorylationGeneticsBiologyChemistryCell biologyBiochemistryYeastDNA Repair MechanismsPlant Genetic and Mutation StudiesPlant Stress Responses and Tolerance