High prevalence of enterotoxigenic Escherichia coli strains in hospitalized diarrhea patients: a preliminary study from a cholera-endemic area in India (2022)
Hemant Kumar Khuntia, Prabir Manna, Deepak Kumar Barik, Subhojeet Biswas, Prasanta Kumar Bramha, Sanghamitra Pati, Manoranjan Ranjit, Madhusmita Bal, Anna Salomi Kerketta
Abstract
Introduction Enterotoxigenic Escherichia coli (ETEC), producing heat-stable (ST) and/or heat-labile (LT) enterotoxins, is a major cause of diarrhea in children and travelers in developing countries. Surveillance in cholera-endemic regions is crucial for timely public health response. Methods Between May and November 2022, a cross-sectional study was conducted in Puri, India. Rectal swabs from 256 hospitalized diarrhea patients were analyzed for diarrheagenic E. coli and Vibrio cholerae using microbiological and molecular methods. Antimicrobial susceptibility of ETEC isolates was also assessed. Results ETEC was detected in 20.3% of cases, making it the most prevalent pathogen. No Vibrio cholerae was isolated. EAEC and EPEC were identified in 6.2% and 2.3% of cases, respectively. ETEC was significantly more common in children under 2 years ( p < 0.00001), though overall age correlation was weak ( R = –0.013). Symptoms included watery stool, vomiting, abdominal pain, and dehydration, with dehydration significantly associated with adults ( p < 0.05). ETEC strains were susceptible to streptomycin, chloramphenicol, gentamicin, and amikacin. Discussion ETEC has emerged as the dominant diarrheal pathogen in a cholera-endemic area, posing a risk to both children and adults. These findings highlight the need for continued epidemiological monitoring and age-targeted interventions.