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Homeostatic control of energy metabolism by monocyte-derived macrophages

Rui Martins, Birte Blankehaus, Faouzi Braza, Miguel Mesquita, Pedro Ventura, Sumnima Singh, Sebastian Weis, Maria V. Pires, Sara Pagnotta, Qian Wu, Sílvia Cardoso, Elisa Jentho, Ana Figueiredo, Pedro Faísca, Ana Nóvoa, Vanessa A. Morais, Stefanie K. Wculek, David Sancho, Moisés Mallo, Miguel P. Soares

2025The EMBO Journal5 citationsDOIOpen Access PDF

Abstract

Multicellular organisms rely on inter-organ communication networks to maintain vital parameters within a dynamic physiological range. Macrophages are central to this homeostatic control system, sensing and responding to deviations of those parameters to sustain organismal homeostasis. Here, we demonstrate that dysregulation of iron (Fe) metabolism, imposed by the deletion of ferritin H chain (FTH) in mouse parenchymal cells, is sensed by monocyte-derived macrophages. In response, monocyte-derived macrophages support tissue function, energy metabolism, and thermoregulation via a mechanism that sustains the mitochondria of parenchymal cells. Mechanistically, FTH supports a transcriptional program promoting mitochondrial biogenesis in macrophages, involving mitochondrial transcription factor A (TFAM). Moreover, FTH sustains macrophage viability and supports intercellular mitochondrial transfer from donor parenchymal cells. In conclusion, monocyte-derived macrophages cross-regulate iron and energy metabolism to support tissue function and organismal homeostasis.

Topics & Concepts

BiologyCell biologyMitochondrionMulticellular organismTranscription factorMacrophageBiogenesisFerritinHomeostasisMitochondrial biogenesisIntracellularFunction (biology)Energy metabolismMetabolismParenchymaRegulation of gene expressionBioenergeticsDownregulation and upregulationTranscription (linguistics)Energy homeostasisSignal transductionCellular respirationThermoregulationTranscriptional regulationIron Metabolism and DisordersMetalloenzymes and iron-sulfur proteinsImmune cells in cancer
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