Evaluation of the Antibacterial Effects of Single and Combined use of Different Irrigation Solutions Against Intracanal Enterococcus Faecalis
Hatice Büyüközer Özkan, Funda Kont Çobankara, Zafer Sayın, Füsun Özer
Abstract
Objectives: This study assessed the antibacterial activity of both separate and combined uses of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 17% ethylenediaminetetraacetic acid (EDTA), 3% hydrogen peroxide (H 2 O 2 ), MTAD, SmearClear (SC) and 13.8% chlorine dioxide (ClO 2 ) irrigation solutions against Enterococcus faecalis. Material and Methods: Two hundred eighty single rooted human premolars were randomly grouped into 26 test and 2 control (negative and positive) groups and were incubated for 24 h with E. faecalis, except for the negative control group. The tested solutions were as follow: NaOCl; CHX; ClO 2 ; MTAD; SC; EDTA; H 2 O 2 ; NaOCl + CHX; NaOCl + MTAD; SC + NaOCl; EDTA + NaOCl; H 2 O 2 + NaOCl; ClO 2 + CHX; CHX + MTAD; SC + CHX; EDTA + CHX; CHX + H 2 O 2 ; ClO 2 + MTAD; SC + ClO 2 ; EDTA + ClO 2 ; ClO 2 + H 2 O 2 ; SC+MTAD; EDTA+MTAD; MTAD + H 2 O 2 ; SC + H 2 O 2; and EDTA + H 2 O 2. Optic density values were recorded at 0, Results: The CHX, MTAD and ClO 2 showed a high potential for the elimination of E. faecalis, both alone and in all combinations. The EDTA, H 2 O 2 , H 2 O 2 + EDTA, H 2 O 2 + NaOCl and SC + NaOCl groups showed less antibacterial activity than the other groups. The SC + CHX group showed the best antibacterial effect against E. faecalis. Conclusion: The SC + CHX combination can be recommended as the most effective irrigation regimen against E. faecalis in persistent endodontic infections.