A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics
Arthur Viodé, Patrick van Zalm, Kinga K. Smolen, Benoit Fatou, David K. Stevenson, Meenakshi Jha, Ofer Levy, Judith A. Steen, Hanno Steen, On behalf of the IMPACC Network
Abstract
We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.
Topics & Concepts
ThroughputComputer scienceProfiling (computer programming)TelecommunicationsOperating systemWirelessAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsAdvanced Biosensing Techniques and Applications