Formulation, Characterization, and Evaluation of Doxorubicin-loaded Cubosome as a Cytotoxic Potentiator against HCT-116 Colorectal Cancer Cells
Yosif Almoshari, Mohammad Intakhab Alam, Mohammed Ali Bakkari, Ahmad Salawi, Meshal Alshamrani, Fahad Y. Sabei, Awaji Y. Safhi, Jawaher Abdullah Alamoudi, Ibrahim M. Ibrahim
Abstract
Background: Colorectal cancer (CRC) has grown to be the world's fourth-biggest cause of cancer-related mortality. It is critical to identify more effective treatment options for it. Objectives: Doxorubicin a potent antineoplastic agent is widely used but has severe adverse effects. Therefore, our objective is to use cubosome as an efficient drug delivery system to reduce the off-target effects and increase the cytotoxic effects in CRC cells. Materials and Methods: Pluronic F127-Based Cubosomes were prepared by low energy stirring method and loaded with doxorubicin. Physicochemical characteristics were studied using X-ray diffraction, dynamic light scattering, microscopic techniques, FTIR UV spectrophotometer, and entrapment efficiency. In vitro activity of doxorubicin-loaded cubosomes was studied on HCT-116 cells. Results: The prepared cubosomes have the required nano-size and the FTIR results showed the presence of both doxorubicin and Pluronic F-127. The entrapment efficiency of doxorubicin was high too. DOX-CBs have a better cytotoxic effect and induced ROS and reduced NO levels in HCT-116 cells.