TEM1 combinatorially binds to <i>FLOWERING LOCUS T</i> and recruits a Polycomb factor to repress the floral transition in <i>Arabidopsis</i>
Hongmiao Hu, Shu Tian, Guohui Xie, Rui Liu, Nana Wang, Sisi Li, Yuehui He, Jiamu Du
Abstract
Significance TEM1 is a flowering repressor that suppresses the expression of FT florigen. However, the specific FT targeting mechanism of TEM1 and the molecular basis for the repression function of TEM1 remain unclear. In this research, we performed structural, biochemical, and genetic studies on TEM1. We revealed the molecular mechanism underlying the combinatorial binding of the FT gene 5′-untranslated region (UTR) by TEM1 AP2 and B3 domains. We found that TEM1 interacts with the PRC2 subunit CLF and is responsible for deposition of the repressive histone mark H3K27me3 at the FT 5′-UTR region. Altogether, we revealed a molecular mechanism underlying the flowering repression function of TEM1.