Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
Jaspreet Osan, Beth Ann DeMontigny, Masfique Mehedi
Abstract
Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differentiation of NHBE cells in air-liquid interface mimics an in vivo airway and provides an in vitro model for studying SARS-CoV-2 infection. We also describe a protocol for detecting proteins in the sectioned epithelium for detailing SARS-CoV-2 infection-induced pathobiology with a vertical view.
Topics & Concepts
Respiratory epitheliumEpitheliumImmunohistochemistryCOPDAirwayLungPathologyIn vivoBiologyIn vitroImmunologyPulmonary diseaseMedicineBiochemistryBiotechnologyPsychiatryInternal medicineSurgeryRespiratory viral infections researchRespiratory Support and MechanismsNeonatal Respiratory Health Research