Maternal Bacterial Engraftment in Multiple Body Sites of Cesarean Section Born Neonates after Vaginal Seeding—a Randomized Controlled Trial
Noel T. Mueller, Moira K. Differding, Haipeng Sun, Jincheng Wang, Shira Levy, Varsha Deopujari, Lawrence J. Appel, Martin J. Blaser, Tanima Kundu, Ankit A. Shah, Maria Gloria Domínguez-Bello, Suchitra K. Hourigan
Abstract
Children delivered by elective C-section are not exposed to the birth canal and show altered microbiota development. Impairing microbial colonization during early life alters metabolic and immune programming and is associated with an increased risk of immune and metabolic diseases. In a double-blind, randomized, placebo-controlled trial, we determined the effect of vaginal seeding on the skin and stool microbiota of elective C-section born neonates and found that vaginal seeding increased mother-to-neonate microbiota transmission and caused compositional changes and a reduction in the skin and stool microbiota diversity. The reduction of neonatal skin and stool microbiota diversity when maternal vaginal microbiota is provided is intriguing and highlights the need of larger randomized studies to determine the ecological mechanisms and effects of vaginal seeding on clinical outcomes.