Dual Photoisomerization on Distinct Potential Energy Surfaces in a UV-Absorbing Rhodopsin
Yusaku Hontani, Matthias Broser, Meike Luck, Jörn Weißenborn, Miroslav Kloz, Peter Hegemann, John T. M. Kennis
Abstract
, after relaxation to the ground state. These isomers become protonated in 58 μs and 3.2 ms, respectively, resulting in formation of the blue-absorbing form of HKR1. Our results constitute a benchmark of UV-induced photochemistry of animal and microbial rhodopsins.
Topics & Concepts
ChemistryPhotoisomerizationGround statePhotochemistryIsomerizationRhodopsinExcited stateProtonationUltrafast laser spectroscopyPhotoexcitationSpectroscopySchiff baseHalorhodopsinDark stateAbsorption spectroscopyCrystallographyRetinalAtomic physicsMembraneBacteriorhodopsinPhysicsOpticsIonOrganic chemistryQuantum mechanicsBiochemistryCatalysisPhotoreceptor and optogenetics researchNicotinic Acetylcholine Receptors Studybioluminescence and chemiluminescence research