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UCell and pyUCell: single-cell gene signature scoring for R and Python

Massimo Andreatta, Santiago J. Carmona

2026Bioinformatics8 citationsDOIOpen Access PDF

Abstract

SUMMARY: Gene signature scoring provides a simple yet powerful approach for quantifying biological signals within single-cell omics datasets. UCell and pyUCell offer fast and robust implementations of rank-based signature scoring for R and Python, respectively, integrating seamlessly with leading single-cell analysis ecosystems such as Seurat, Bioconductor, and scanpy/scverse. AVAILABILITY AND IMPLEMENTATION: UCell v2 is distributed as an R package by BioConductor (https://bioconductor.org/packages/UCell/) and as a Python package by pyPI (https://pypi.org/project/pyucell/).

Topics & Concepts

Python (programming language)BioconductorComputer scienceR packageProgramming languageSignature (topology)Computational biologyOpen sourceArtificial intelligenceSoftwareData miningSoftware packageAlgorithmSingle-cell and spatial transcriptomicsFerroptosis and cancer prognosisGenomic variations and chromosomal abnormalities
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