Fluorescent Protein Expression as a Proxy for Bacterial Fitness in a High-Throughput Assay
R. Schlechter, Evan J. Kear, Daniela M. Remus, Mitja N. P. Remus‐Emsermann
Abstract
We expand on an important limitation of the concept of measuring bacterial growth, which is classically limited to one strain at a time. By adopting our approach, it is possible to measure the growth of several bacterial strains simultaneously with high temporal resolution and in a high-throughput manner. This is important to investigate bacterial interactions, such as competition and facilitation.
Topics & Concepts
Bacterial growthProxy (statistics)BiologyThroughputBacterial proteinHigh-throughput screeningFluorescenceProtein expressionBacterial strainComputational biologyBacteriaBioinformaticsGeneticsComputer scienceGeneMachine learningWirelessTelecommunicationsQuantum mechanicsPhysicsAdvanced Fluorescence Microscopy TechniquesBacterial Genetics and BiotechnologyCell Image Analysis Techniques