An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
Ruiwen Ding, Jianxi Ying, Yufen Zhao
Abstract
Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.
Topics & Concepts
Circular dichroismEnantiomerChemistryChromatographyAbsolute configurationEnantiomeric excessChirality (physics)Amino acidOptical rotationMoleculeCalibration curveQuantitative analysis (chemistry)Amino acid analysisAnalytical Chemistry (journal)StereochemistryOrganic chemistryChiral symmetryPhysicsEnantioselective synthesisBiochemistryDetection limitQuarkNambu–Jona-Lasinio modelQuantum mechanicsCatalysisDNA and Nucleic Acid ChemistryMolecular spectroscopy and chiralityAnalytical Chemistry and Chromatography