Chemical synthesis of human selenoprotein F and elucidation of its thiol-disulfide oxidoreductase activity
Peisi Liao, Hongmei Liu, Chunmao He
Abstract
, thus facilitating the synthesis of multi-milligrams of homogenous SelF. The reduction potential of SelF was determined and the thiol-disulfide oxidoreductase activity was further supported by its ability to catalyze the reduction and isomerization of disulfide bonds.
Topics & Concepts
SelenoproteinThiolOxidoreductaseDisulfide bondSelenoprotein PChemistryStereochemistryBiochemistryCombinatorial chemistryEnzymeGlutathioneGlutathione peroxidaseProtein Hydrolysis and Bioactive PeptidesRedox biology and oxidative stressSelenium in Biological Systems