A novel fluorescence amplification strategy combining cascade primer exchange reaction with CRISPR/Cas12a system for ultrasensitive detection of RNase H activity
Zuowei Xie, Siyi Chen, Wenxiu Zhang, Shuhui Zhao, Zixin Zhao, Xingyu Wang, Yuqi Huang, Gang Yi
Topics & Concepts
RNase HRNase PPrimer (cosmetics)NucleaseCRISPRRibonucleaseDNAChemistryDetection limitMolecular biologyLoop-mediated isothermal amplificationRNAOligonucleotideDeoxyribozymeBiochemistryBiologyChromatographyGeneOrganic chemistryCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery