Cryo-OrbiSIMS for 3D Molecular Imaging of a Bacterial Biofilm in Its Native State
Junting Zhang, James Brown, David J. Scurr, Anwen Bullen, Kirsty MacLellan‐Gibson, Paul Williams, Morgan R. Alexander, Kim R. Hardie, Ian S. Gilmore, Paulina D. Rakowska
Abstract
biofilm in its native state. The 3D distribution of quorum sensing signaling molecules, nucleobases, and bacterial membrane molecules is revealed with high spatial-resolution and high mass-resolution. We discover that analysis in the frozen-hydrated state yields a 10 000-fold increase in signal intensity for polar molecules such as amino acids, which has important implications for SIMS imaging of metabolites and pharmaceuticals.
Topics & Concepts
ChemistryMass spectrometry imagingMass spectrometryOrbitrapSecondary ion mass spectrometryBiofilmMolecular imagingMoleculeAnalytical Chemistry (journal)MALDI imagingQuorum sensingBiophysicsChromatographyMatrix-assisted laser desorption/ionizationBacteriaBiochemistryBiotechnologyBiologyDesorptionAdsorptionOrganic chemistryGeneVirulenceGeneticsIn vivoIon-surface interactions and analysisBacterial biofilms and quorum sensingBacterial Identification and Susceptibility Testing