Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling
Yiran Huang, Yuqing Deng, Jianfu Zhang, Ling Meng, Xiaoyu Li
Abstract
Membrane proteins are important drug targets; however, ligand discovery for membrane proteins is highly challenging due to their hydrophobic nature. We show that membrane proteins may be specifically labelled with a DNA tag by DNA-programmed affinity labelling (DPAL), thereby enabling the screening of chemical compounds against membrane proteins directly on live cells.
Topics & Concepts
LabellingLigand (biochemistry)DNAChemistryAffinity labelMembrane proteinMembraneAffinity labelingDNA-binding proteinComputational biologyBiochemistryCombinatorial chemistryBiologyBinding siteReceptorGeneTranscription factorClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniquesMonoclonal and Polyclonal Antibodies Research