Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids
Mito Kanatsu-Shinohara, Narumi Ogonuki, Shogo Matoba, Hiroko Morimoto, Yusuke Shiromoto, Atsuo Ogura, Takashi Shinohara
Abstract
donor cells colonized both types of transplants and produced sperm. Moreover, rat embryonic gonads supported xenogeneic spermatogenesis from mouse SSCs when transplanted in testes of immunodeficient mice. Offspring with normal genomic imprinting patterns were born after microinsemination. These results demonstrate remarkable flexibility of the germ cell-somatic cell interaction and raise new strategies of SSC manipulation for animal transgenesis and analysis of male infertility.
Topics & Concepts
BiologySpermatogenesisTransgenesisSomatic cellGerm cellTransplantationCell biologyEmbryonic stem cellGermlineStem cellReproductive technologySpermEmbryoAndrologyImmunologyGeneticsEmbryogenesisInternal medicineEndocrinologyGeneMedicineSperm and Testicular FunctionReproductive Biology and FertilityGenetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities