Simple Phenotypic Tests To Improve Accuracy in Screening Chromosomal and Plasmid-Mediated Colistin Resistance in Gram-Negative Bacilli
Fernando Pasterán, Diego Danze, Alejandra Menocal, Carla Cabrera, Ignacio del Castillo, Ezequiel Albornoz, Celeste Lucero, Melina Rapoport, Paola Ceriana, Alejandra Corso
Abstract
CLSI and EUCAST recommend that only broth microdilution (BMD) should be used for routine colistin susceptibility testing; however, this technique can be difficult to perform in resource-poor settings. The purpose of this study was to evaluate the accuracy of a colistin agar spot test (COL-AS) and a colistin drop test (COL-DT) compared to BMD. COL-AS and COL-DT were assessed with a collection of 271 Gram-negative bacilli clinical isolates: 195 Enterobacterales (including 63 mcr-1 positive strains), 37 Acinetobacter spp.
Topics & Concepts
ColistinGramPlasmidBiologyPhenotypeMicrobiologyBacilliGram-negative bacteriaGram-negative bacterial infectionsGeneticsBacteriaAntibioticsGeneEscherichia coliAntibiotic Resistance in BacteriaEnterobacteriaceae and Cronobacter ResearchSalmonella and Campylobacter epidemiology