TRPV4 and chloride channels mediate volume sensing in trabecular meshwork cells
Jackson M. Baumann, Oleg Yarishkin, Mónika Lakk, Michael L. De Ieso, Christopher N. Rudzitis, Megan Kuhn, Yun‐Ting Tseng, W. Daniel Stamer, David Križaj
Abstract
Intraocular pressure is dynamically regulated by the flow of aqueous humor through paracellular passages within the trabecular meshwork (TM). This study shows hypotonic gradients that expand the TM cell volume and reduce the outflow facility in mouse eyes. The swelling-induced current consists of TRPV4 and chloride components, with TRPV4 as a driver of swelling-induced calcium signaling. TRPV4 inhibition reduced swelling, suggesting a novel treatment for trabeculitis and glaucoma.
Topics & Concepts
Trabecular meshworkTRPV4Chloride channelCell biologyVolume (thermodynamics)BiophysicsChemistryMaterials scienceNeuroscienceBiologyGlaucomaIon channelPhysicsBiochemistryQuantum mechanicsReceptorIon Channels and ReceptorsPain Mechanisms and TreatmentsConnexins and lens biology