Protein Profiling of Resistant and Susceptible Mutant Lines of Rice Variety Swarna in Response to Rhizoctonia Solani AG1 IA Infection
Reeshu Singh, Sumant Pratap Singh, Ankit Singh, Prabhat Kumar Singh, D. K. Dwivedi, Nafees A. Khan
Abstract
Rice sheath blight, caused by Rhizoctonia solani, is one of the most devastating diseases for stable rice production in most rice-growing regions of the world. Currently, studies of the molecular mechanism of rice sheath blight resistance are scarce. Here, we used mutated rice population of the variety swarna induced by the sodium azide (NaN3). The variability in disease reaction was observed among mutated rice lines. Out of the total 1000 mutant plants, 47 plants were screened that show low disease index, ranged between 0 to 5 were selected for next generation. In M1 generation the selected genotypes show differences for disease index, only 12 lines shows resistance in M 1 generation in field condition and in humidity chamber condition only 8 lines shows resistance. The protein analysis by SDS-PAGE showed that the resistance lines have one extra band in between 33 kDa and 43 kDa that is not showing in moderate and susceptible lines. Thus, these resistance lines could be considered a potential source for disease resistance against the sheath blight of rice and could be used further in the crossing programme for development of sheath blight resistant rice variety.