Comparison of Genome and Plasmid-Based Engineering of Multigene Benzylglucosinolate Pathway in Saccharomyces cerevisiae
Cuiwei Wang, Michal Poborsky, Christoph Crocoll, Christina Spuur Nødvig, Uffe Hasbro Mortensen, Barbara Ann Halkier
Abstract
) in the biosynthetic pathway, the genome-engineered strain produced higher production level of benzylglucosinolate. Based on the genome-engineered strain, the benzylglucosinolate level was improved by optimization. Our study compared different approaches to engineer a multigene pathway for production of the plant natural product benzylglucosinolate. This may provide potential application in industrial biotechnology.
Topics & Concepts
Saccharomyces cerevisiaePlasmidGenomeBiologyGeneticsComputational biologyGeneGenomics, phytochemicals, and oxidative stressFungal and yeast genetics researchPhytochemicals and Antioxidant Activities