Isolation, Characterization and<i>in vitro</i>Evaluation of Specific Bacteriophages Targeting Extensive Drug Resistance Strains of<i>Pseudomonas aeruginosa</i>Isolated from Septic Burn Wounds
Seyed Mahmoud Amin Marashi, Farhad Nikkhahi, Dariush Hamedi, Gholamhassan Shahbazi
Abstract
BACKGROUND: Antibiotic resistant bacteria and various infections caused by them especially extensive drug resistance (XDR) strains and worrying statistics of mortality due to these strains and also the lack of a clear vision for development and production of new effective antibiotics have made the necessity of using alternative therapies more apparent. MATERIALS AND METHODS: were investigated. RESULTS: strains. More than 98% of phages absorbed their host in less than 10 minutes (Adsorption time <10 min) and completed their lytic cycle after 40 minutes (latent time = 40 min). Burst size of PaBa1, PaBa2 and PaBa3 was 240, 250 and 220 pfu/cell, respectively. PaBa1 lysed 62.5% of the XDR strains with the highest efficiency. The three Phage cocktail was effective against 67.5% of the studied strains. CONCLUSION: The results of this study indicate the significant potential of these phages for therapeutic use and prophylaxis of infections caused by this bacterium.