Mesenchymal Stem Cells cause Telomere Length Reduction of Molt-4 Cells via Caspase-3, BAD and P53 Apoptotic Pathway.
Hamid Heidari, Ezzatollah Fathi, Soheila Montazersaheb, Ayoub Mamandi, Raheleh Farahzadi, Soran Zalavi, Hojjatollah Nozad Charoudeh
Abstract
gene expression of Molt-4 cells. Also, gene and protein expression of BAD and P53 were significantly increased. Furthermore, the flow cytometry analysis indicated the decrease and increase of the Ki-67 and caspaspase-3 expression, respectively. It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis via caspase-3, BAD, and P53 expression. In addition, the decrease of telomere length is another effect of MSCs on Molt-4 leukemic cells.
Topics & Concepts
Mesenchymal stem cellTelomereFlow cytometryApoptosisMolecular biologyBiologyStem cellCancer researchReal-time polymerase chain reactionCell biologyGeneGeneticsMesenchymal stem cell researchMicroRNA in disease regulation