Quantitative Mass Spectrometry Imaging of Biological Systems
Daisy Unsihuay, Daniela Mesa Sanchez, Julia Laskin
Abstract
Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed maps of hundreds of molecules in complex samples with high sensitivity and subcellular spatial resolution. Accurate quantification in MSI relies on a detailed understanding of matrix effects associated with the ionization process along with evaluation of the extraction efficiency and mass-dependent ion losses occurring in the analysis step. We present a critical summary of approaches developed for quantitative MSI of metabolites, lipids, and proteins in biological tissues and discuss their current and future applications.
Topics & Concepts
Mass spectrometryMass spectrometry imagingChemistryExtraction (chemistry)Biological systemComputational biologyChromatographyBiologyMass Spectrometry Techniques and ApplicationsAnalytical Chemistry and ChromatographyMetabolomics and Mass Spectrometry Studies