Spherical nucleic acid reporter-based cascade CRISPR/Cas12a amplifier for stable and sensitive biosensing of circulating tumor DNA
Min Zhou, Yao Yin, Yuyan Shi, Zhaoxin Huang, Yu Shi, Mei Chen, Guoliang Ke, Xiaobing Zhang
Abstract
Stable and sensitive ctDNA biosensing in complex biological fluid is highly important but still remains a challenge. Herein, we develop a spherical nucleic acid reporter-based cascade CRISPR/Cas12a amplifier with improved stability and sensitivity (5 orders of magnitude).
Topics & Concepts
Nucleic acidBiosensorCascadeCRISPRDNAChemistryNanotechnologyMaterials scienceBiochemistryChromatographyGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesSingle-cell and spatial transcriptomics