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LncRNA HULC shRNA disinhibits miR-377-5p to suppress the growth and invasion of hepatocellular carcinoma in vitro and hepatocarcinogenesis in vivo

Chunxiao Yan, Shutang Wei, Dazheng Han, Liping Wu, Lixia Tan, Hangyu Wang, Yong Dong, Jing Hua, Wen‐Yi Yang

2020Annals of Translational Medicine22 citationsDOIOpen Access PDF

Abstract

BACKGROUND: Aberrant expression of up-regulated long non-coding RNA [LncRNA highly upregulated in liver cancer (HULC)] has been observed to play an important regulatory role in the development of multiple human diseases. However, the molecular mechanism underlying the role of HULC and miR-377-5p in HCC needs to be urgently explored. METHODS: . RESULTS: experiments indicated that down-regulation of HULC could inhibit tumor growth. Taken together, our research demonstrated that down-regulation of HULC plays an anti-cancer role through restrainingHepG2 cell proliferation and invasion. CONCLUSIONS: findings confirmed HULC to play a role in the progression of HCC, with the underlying mechanism possibly involving the miR-377-5p/HIF-1α pathway.

Topics & Concepts

Small hairpin RNAmicroRNAWestern blotTransfectionHepatocellular carcinomaCell growthCancer researchMolecular biologyBiologyIn vivoDownregulation and upregulationCell cultureGene knockdownGeneGeneticsCancer-related molecular mechanisms researchCancer Mechanisms and TherapyCircular RNAs in diseases
LncRNA HULC shRNA disinhibits miR-377-5p to suppress the growth and invasion of hepatocellular carcinoma in vitro and hepatocarcinogenesis in vivo | Litcius