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Mass spectrometry-based proteomics data from thousands of HeLa control samples

Henry Webel, Yasset Pérez‐Riverol, Annelaura Bach Nielsen, Simon Rasmussen

2024Scientific Data11 citationsDOIOpen Access PDF

Abstract

Here we provide a curated, large scale, label free mass spectrometry-based proteomics data set derived from HeLa cell lines for general purpose machine learning and analysis. Data access and filtering is a tedious task, which takes up considerable amounts of time for researchers. Therefore we provide machine based metadata for easy selection and overview along the 7,444 raw files and MaxQuant search output. For convenience, we provide three filtered and aggregated development datasets on the protein groups, peptides and precursors level. Next to providing easy to access training data, we provide a SDRF file annotating each raw file with instrument settings allowing automated reprocessing. We encourage others to enlarge this data set by instrument runs of further HeLa samples from different machine types by providing our workflows and analysis scripts.

Topics & Concepts

Computer scienceWorkflowMetadataSet (abstract data type)Scripting languageRaw dataFile formatData setDatabaseMass spectrometryProteomicsInformation retrievalData miningOperating systemChemistryProgramming languageArtificial intelligenceChromatographyBiochemistryGeneAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies
Mass spectrometry-based proteomics data from thousands of HeLa control samples | Litcius