The m6A demethylase fat mass and obesity-associated protein mitigates pyroptosis and inflammation in doxorubicin-induced heart failure via the toll-like receptor 4/NF-κB pathway
Weiling Tu, Xiao Huang, Song‐Tao Liu, Yuliang Zhan, Xinyong Cai, Liang Shao
Abstract
Background: A) demethylase fat mass and obesity-associated protein (FTO) in pyroptosis and inflammation during Dox-induced heart failure (HF). Methods: A methylation levels. The expression of p-NF-κB p65 and p-IκB-α was measured by western blotting. Results: A demethylation of TLR4, thus declining TLR4, p-NF-κB p65, and p-IκB-α expression. TLR4 knockdown counteracted the effects of FTO knockdown on Dox-induced H9C2 cells. Conclusions: FTO alleviated Dox-induced HF by blocking the TLR4/NF-κB pathway.
Topics & Concepts
PyroptosisGene knockdownTLR4InflammationApoptosisWestern blotMedicineReceptorMolecular biologyPharmacologyCancer researchChemistryBiologyInternal medicineInflammasomeBiochemistryGeneRNA modifications and cancerHVDC Systems and Fault ProtectionCardiac Structural Anomalies and Repair