A light-activated polymer with excellent serum tolerance for intracellular protein delivery
Lanfang Ren, Li Jiang, Qianyi Ren, Jia Lv, Linyong Zhu, Yiyun Cheng
Abstract
ionic interactions, followed by light activation to yield aldehyde groups on the dendrimer and the formation of imine bonds with cargo proteins. The light-activated complexes show high stability in buffer and serum solutions, but dis-assemble under low pH conditions. As a result, the polymer successfully delivers cargo proteins green fluorescent protein and β-galactosidase into cells with maintained bioactivity even in the presence of 50% serum. The LAC strategy proposed in this study provides a new insight to improve the serum stability of polymers for intracellular protein delivery.
Topics & Concepts
DendrimerIntracellularChemistryBiophysicsCationic polymerizationPolymerImineGreen fluorescent proteinCombinatorial chemistryBiochemistryPolymer chemistryOrganic chemistryBiologyCatalysisGeneRNA Interference and Gene DeliveryPhotochromic and Fluorescence ChemistryDendrimers and Hyperbranched Polymers