A perylene diimide-based nanoring architecture for exogenous and endogenous ATP detection: biochemical assay for monitoring phosphorylation of glucose
Prabhpreet Singh, Poonam Sharma, Neha Sharma, Satwinderjeet Kaur
Abstract
electrostatic and H-bonding interactions. The complexation mechanism of PDI 3 and ATP was confirmed by optical, NMR, Job's plot, DLS, SEM and AFM studies. PDI 3 displays low cytotoxicity toward MG-63 cells and can be successfully used for the detection of exogenous and endogenous ATP. The resulting PDI 3 + ATP complex is successfully used as a 'turn-on' biochemical assay for monitoring phosphorylation of glucose.
Topics & Concepts
PeryleneDiimidePhosphorylationEndogenyMaterials scienceBiochemistryBiologyChemistryOrganic chemistryMoleculeAdvanced biosensing and bioanalysis techniquesElectrochemical sensors and biosensorsAnalytical Chemistry and Sensors