Electrochemical Detection of Single-Nucleotide Polymorphism Associated with Rifampicin Resistance in <i>Mycobacterium tuberculosis</i> Using Solid-Phase Primer Elongation with Ferrocene-Linked Redox-Labeled Nucleotides
Mayreli Ortiz, Miriam Jauset‐Rubio, Vasso Skouridou, Diana Machado, Miguel Viveiros, Taane G. Clark, Anna Šimonová, David Kodr, Michal Hocek, Ciara K. O’Sullivan
Abstract
strains. The approach is rapid, cost-effective, facile, and can be extended to multiplexed electrochemical single-point mutation genotyping.
Topics & Concepts
Primer (cosmetics)OligonucleotidePrimer extensionMycobacterium tuberculosisNucleotideDNAChemistryMolecular biologyBiologyBiochemistryTuberculosisOrganic chemistryGeneMedicinePathologyAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry