MicroRNA-202 suppresses glycolysis of pancreatic cancer by targeting hexokinase 2
Shuangjia Wang, Xiu-Dong Li, Lupeng Wu, Ping Guo, Liuxing Feng, Bin Li
Abstract
Purpose: Various studies have identified miR-202 critically participated in the development of different cancers. However, the potential mechanisms underlying the carcinogenesis of pancreatic cancer (PC) still remains elusive. Methods: In the study, cell proliferation assay, colony formation assay, EdU incorporation assay, Luciferase reporter assay, lactate production, glucose consumption assay, real-time PCR and western blot were used to investigate the mechanism of hexokinase 2 (HK2) regulated by miR-202 in pancreatic cancer in vitro and in vivo.
Topics & Concepts
HexokinaseGlycolysismicroRNAPancreatic cancerCancerCancer researchMedicineChemistryCell biologyBiochemistryBiologyInternal medicineEnzymeGeneCancer, Hypoxia, and MetabolismBiochemical and Molecular ResearchPancreatic function and diabetes