Immunomodulatory effects of the polysaccharide from <i>Sinonovacula constricta</i> on RAW264.7 macrophage cells
Zhidong Liu, Zhidong Liu, Zhifang Liu, Zhifang Liu, Chunsheng Li, Junjie Zhang, Qiancheng Zhao, Na Lin, Wenzhu Zhong, Mei Jiang
Abstract
Abstract This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP‐1‐1) in RAW264.7 cells. SCP‐1‐1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP‐1‐1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF‐α, IL‐6, and IL‐1β) secretion of RAW264.7 cell in a dose‐dependent manner. Western blot analysis results demonstrated that SCP‐1‐1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF‐κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta .