Development of a Measles and Rubella Multiplex Bead Serological Assay for Assessing Population Immunity
Melissa M. Coughlin, Zachary Matson, Sun B. Sowers, Jeffrey W. Priest, Gaby Smits, Fiona van der Klis, Alexandria Mitchell, Carole J. Hickman, Heather M. Scobie, James L. Goodson, James P. Alexander, Paul A. Rota, Bettina Bankamp
Abstract
= 0.919). ROC analysis of the rubella MBA using ELISA as the comparator yielded a cutoff of 9.36 IU/ml, similar to the accepted cutoff of 10 IU/ml for seroprotection, with a sensitivity of 99% and a specificity of 100%. These results support use of the MBA for multiantigen serosurveys assessing measles and rubella population immunity.
Topics & Concepts
RubellaSerologyMultiplexMeaslesVirologyImmunityBiologyPopulationImmunologyMedicineVaccinationAntibodyImmune systemGeneticsEnvironmental healthVirology and Viral DiseasesSARS-CoV-2 and COVID-19 ResearchZoonotic diseases and public health