Mass spectrometry reveals the assembly pathway of encapsulated ferritins and highlights a dynamic ferroxidase interface
Jennifer Ross, Thomas Lambert, Cecilia Piergentili, Didi He, Kevin J. Waldron, C. Logan Mackay, Jon Marles‐Wright, David J. Clarke
Abstract
Encapsulated ferritins (EncFtn) are a recently characterised member of the ferritin superfamily. EncFtn proteins are sequestered within encapsulin nanocompartments and form a unique biological iron storage system. Here, we use native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to elucidate the metal-mediated assembly pathway of EncFtn.
Topics & Concepts
Mass spectrometryChemistryFerritinCeruloplasminHydrogen–deuterium exchangeBiochemistryChromatographyIron Metabolism and DisordersRNA and protein synthesis mechanismsTrace Elements in Health