Determination of glyphosate and AMPA in freshwater and soil from agroecosystems by 9-fluorenylmethoxycarbonyl chloride derivatization and liquid chromatography - fluorescence detection and tandem mass spectrometry
Beatriz Alonso, Luciana Griffero, Heinkel Bentos Pereira, Lucía Pareja, Andrés Pérez‐Parada
Abstract
The herbicide glyphosate (GLY) and its metabolite aminophosphonic acid (AMPA) are troublesome compounds for analysis in the environment. Here we report a reliable technique for GLY and AMPA determination in freshwater and soils by means of derivatization with 9-fluorenylmethoxycarbonyl chloride (FMOC-Cl) and further liquid chromatography with fluorescence detection (FLD) and tandem mass spectrometry (MS/MS) analysis. Selected experiments were carried out to evaluate selectivity, sensitivity, repeatability, linearity and quantification performance in both matrices.
Topics & Concepts
DerivatizationChromatographyDansyl chlorideChemistryGlyphosateTandem mass spectrometryMass spectrometryChlorideLiquid chromatography–mass spectrometryHigh-performance liquid chromatographyRepeatabilityAminomethylphosphonic acidMetaboliteOrganic chemistryBiochemistryAgronomyBiologyPesticide and Herbicide Environmental StudiesWeed Control and Herbicide ApplicationsPesticide Exposure and Toxicity