Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from <i>Mycobacterium tuberculosis</i> and Preparation of Its Polyclonal Antibody Protein
Tao Xu, Minying Li, Chutong Wang, Meili Yuan, Xianyou Chang, Zhongqing Qian, Baiqing Li, Meiqun Sun, Hongtao Wang
Abstract
and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.
Topics & Concepts
Polyclonal antibodiesRecombinant DNAFusion proteinMycobacterium tuberculosisAntiserumBiologyMolecular biologyAntibodyTiterAntigenGeneBiochemistryTuberculosisImmunologyPathologyMedicineTuberculosis Research and EpidemiologyBiochemical and Molecular ResearchBacteriophages and microbial interactions