Reconstitution of the destruction complex defines roles of AXIN polymers and APC in β-catenin capture, phosphorylation, and ubiquitylation
Michael Ranes, Mariola Zaleska, Saira Sakalas, Ruth Knight, Sebastian Guettler
Abstract
E3 ligase complex in a β-TrCP-dependent manner. Oncogenic APC truncation variants, although part of the complex, are functionally impaired. Nonetheless, even the most severely truncated APC variant promotes β-catenin recruitment. These findings exemplify the power of biochemical reconstitution to interrogate the molecular mechanisms of Wnt/β-catenin signaling.
Topics & Concepts
BiologyWnt signaling pathwayPhosphorylationCateninCell biologyUbiquitinUbiquitin ligaseImmunoprecipitationBeta-cateninSignal transductionBiochemistryGeneticsCell cultureGeneWnt/β-catenin signaling in development and cancerCancer-related gene regulationUbiquitin and proteasome pathways