Insertional Inactivation and Gene Complementation of Prevotella intermedia Type IX Secretion System Reveals Its Indispensable Roles in Black Pigmentation, Hemagglutination, Protease Activity of Interpain A, and Biofilm Formation
Mariko Naito, Mikio Shoji, Keiko Sato, Koji Nakayama
Abstract
The virulence factors of periodontal pathogens such as Prevotella intermedia have not been elucidated. Using our established procedure, we succeeded in generating type IX secretion system mutants and gene complementation strains that might transfer virulence factors to the bacterial surface. The generated strains clearly indicate that T9SS in P. intermedia is essential for colonial pigmentation, hemagglutination, and biofilm formation. These results indicated that interpain A is a T9SS cargo protein.
Topics & Concepts
Prevotella intermediaBiologyVirulenceComplementationMicrobiologyPorphyromonas gingivalisMutantBacterial outer membraneGeneticsGeneEscherichia coliBacteriaOral microbiology and periodontitis researchBacterial biofilms and quorum sensingBacterial Identification and Susceptibility Testing