NEAT1 regulates VSMC differentiation and calcification in as long noncoding RNA NEAT1 enhances phenotypic and osteogenic switching of vascular smooth muscle cells in atherosclerosis via scaffolding EZH2
Chengye Yin, Zhuowang Ge, Jiali Yuan, Yuhan Chen, Yong Tang, Xiang Yin, Yachen Zhang
Abstract
Our study demonstrates that the upregulation of long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes proliferation and migration during phenotypic switching of vascular smooth muscle cells in atherosclerosis. We also provide in vivo and in vitro evidence that NEAT1 accelerates vascular calcification. Our findings identified the direct interaction between enhancer of zeste homolog 2 (EZH2) and NEAT1 during atherosclerosis. NEAT1 is necessary for EZH2 to translocate from the cytoplasm to the nucleus, where EZH2 epigenetically inhibits the expression of genes related to senescence and antimigration.
Topics & Concepts
Vascular smooth muscleLong non-coding RNAPhenotypePhenotypic switchingCell biologyNon-coding RNAScaffoldEZH2Cancer researchBiologyRNAChemistrySmooth muscleGeneGene expressionMedicineGeneticsBiomedical engineeringEndocrinologyCancer-related molecular mechanisms researchAtherosclerosis and Cardiovascular DiseasesLipid metabolism and disorders