Neotelomeres and telomere-spanning chromosomal arm fusions in cancer genomes revealed by long-read sequencing
Kar‐Tong Tan, Michael K. Slevin, Mitchell L. Leibowitz, Max Garrity-Janger, Jidong Shan, Heng Li, Matthew Meyerson
Abstract
Alterations in the structure and location of telomeres are pivotal in cancer genome evolution. Here, we applied both long-read and short-read genome sequencing to assess telomere repeat-containing structures in cancers and cancer cell lines. Using long-read genome sequences that span telomeric repeats, we defined four types of telomere repeat variations in cancer cells: neotelomeres where telomere addition heals chromosome breaks, chromosomal arm fusions spanning telomere repeats, fusions of neotelomeres, and peri-centromeric fusions with adjoined telomere and centromere repeats. These results provide a framework for the systematic study of telomeric repeats in cancer genomes, which could serve as a model for understanding the somatic evolution of other repetitive genomic elements.