Effect of the Methanol Extract of Annona squamosa Linn Leaf on Cervical Cancer
Made Dira Swantara, Wiwik Susanah, Made Asmarani Dira, Kadek Karang, Al-Ghazzawi Am, Aoac, A Atanasov, B Waltenberger, E-M Pferschy-Wenzig, T Linder, C Wawrosch, P Uhrin, V Temml, L Wang, S Schwaiger, E Heiss, J Rollinger, D Schuster, J Breuss, V Bochkov, M Mihovilovic, B Kopp, R Bauer, V Dirsch, H Stuppner, A Bhattacharya, R Chakraverty, V Biba, A Amily, S Sangeetha, P Remani, K Byju, V Anuradha, G Vasundhara, S Nair, N Kumar, J Carballo, Z Hernndez-Inda, P Prez, Garca-Grvalos Md, G Cragg, D Newman, Odj Dailey, X Wang, Chen Huang, G, D Dias, Urban Roessner, U, A Fadholly, A Proboningrat, Dewi Iskandar, R Rantam, F Sudjarwo, S, M Ganesh, M Mohankumar, C Huang, D Ju, C Chang, Muralidhar Reddy, P Velmurugan, B, B Joy, P Remani, Karan Erenler, R, P Kattner, K Zeiler, V Herbener, K Ferla-Brhl, R Kassubek, M Grunert, T Burster, O Brhl, A Weber, H Strobel, G Karpel-Massler, S Ott, A Hagedorn, D Tews, A Schulz, V Prasad, M Siegelin, L Nonnenmacher, P Fischer-Posovszky, M Halatsch, K Debatin, M Westhoff, S Kauser, S Tasneem, R Yasmeen, L Larmour, Jobling Tw, C Gargett, C Ma, Y Chen, J Chen, Li, Chen
Abstract
Anona squamosa Linn leaves methanol extract was tested for anticancer activity, and the active components were identified in this study. The brine shrimp lethality test (BSLT) was used to measure the chemicals toxicity of the A. squamosa leaves extracted at room temperature using methanol. An anticancer test on HeLa cells was also carried out using this hazardous extract. The identification of the active compounds was carried out by Gas chromatography-mass spectrometry. At an LC50 value of 26.30ppm the extract was toxic, while at 35.65, it showed anticancer activity. Furthermore, 15 compounds which were responsible for this anticancer activity were identified. They include: ethyl-diethylene glycol, 3,7-Dimethyl-6-octenal, 6-Octen-1-ol, 3,7-dimethyl-, 2,6-Octadien-1-ol, 3,7-dimethyl, 1-Methoxy-4- (1-propenyl) benzene, 2,6-Octadien-1-ol, 3,7-dimethyl-, acetate or Geranyl acetate, methyl-dodecanoic acid, methyl-tetradecanoic acid, methyl-hexadecanoic acid, n-Hexadecanoic acid, 7-Acethyl-6-ethyl-1,1,4,4-tetramethyltetralyn, methyl-9-Octadecenoic acid, 9,12-Octamethyl-decadienoic acid, 9,12-Octadecadienoic acid, and bis (2-ethylhexyl)-1,2-Benzenedicarboxylic acid. According to this study, A. squamosa leaves can be employed as a natural anticancer agent for cervical cancer.