Installing CRISPR–Cas12a sensors in a portable glucose meter for point-of-care detection of analytes
Ran Liu, Ying He, Tian Lan, Jingjing Zhang
Abstract
Integrating CRISPR-Cas12a sensors with a portable glucose meter (PGM) was developed based on the target-induced activation of the collateral cleavage activity of Cas12a. Considering the portability, low cost and facile incorporation of the PGM system with suitable Cas12a sensors to recognize many targets, the CRISPR/Cas12a-PGM system demonstrated here paves a way to further broaden the POC applications of CRISPR-based diagnostics.
Topics & Concepts
CRISPRGlucose meterInstallationPoint of carePoint-of-care testingAnalyteComputer scienceComputational biologyChromatographyChemistryOperating systemBiologyMedicineBiochemistryDiabetes mellitusPathologyGeneEndocrinologyAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringBiosensors and Analytical Detection