Iron Fenton oxidation of 2′-deoxyguanosine in physiological bicarbonate buffer yields products consistent with the reactive oxygen species carbonate radical anion not the hydroxyl radical
Aaron M. Fleming, Cynthia J. Burrows
Abstract
Product analysis from the iron Fenton oxidation of 2'-deoxyguanosine found reactions in bicarbonate buffer yield 8-oxo-2'-deoxyguanosine and spiroiminodihyantoin consistent with CO3˙-. Reactions in phosphate buffer furnished high yields of sugar oxidation products consistent with HO˙. These observations change the view of DNA oxidation products from the iron-Fenton reaction.
Topics & Concepts
ChemistryDeoxyguanosineBicarbonateOxidizing agentHydroxyl radicalCarbonateYield (engineering)RadicalPhotochemistryInorganic chemistryOxygenRadical ionReactive oxygen speciesIonNuclear chemistryOrganic chemistryAdductBiochemistryMetallurgyMaterials sciencePorphyrin Metabolism and DisordersAdvanced oxidation water treatmentMetal-Catalyzed Oxygenation Mechanisms