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Downregulation of hsa_circ_0004543 Activates oxLDL-Induced Vascular Endothelial Cell Proliferation and Angiogenesis

Lu Han, Dandan Li, Yanwen Hang, Zong Xiaojuan, Jiashun Lv, Xiaolu Bai, Yi Lü, Peng Zhang, Meiling Zhou, Zhaodi Wu, Wei Hu

2021Frontiers in Genetics16 citationsDOIOpen Access PDF

Abstract

Circular RNAs (circRNAs) are novel non-coding RNAs, which show abnormal expression in several diseases, such as atherosclerosis (AS). The purpose of the present study was to reveal the association between hsa_circ_0004543 and AS. In the present study, hsa_circ_0004543 was overexpressed in human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (oxLDL). Inhibition of hsa_circ_0004543 expression facilitated the proliferation, migration, and invasion of HUVECs and significantly reduced their apoptotic rate following treatment with oxLDL. Furthermore, silencing of hsa_circ_0004543 activated the PI3K/AKT/NOS3 pathway in oxLDL-induced HUVECs. Collectively, these results demonstrated that hsa_circ_0004543 may play a vital role in the development of AS and affect the proliferation of HUVECs, providing a potential target for treating endothelial cell damage in AS.

Topics & Concepts

Umbilical veinAngiogenesisDownregulation and upregulationGene silencingCell growthPI3K/AKT/mTOR pathwayCell biologyChemistryApoptosisProtein kinase BCancer researchBiologySignal transductionBiochemistryIn vitroGeneCircular RNAs in diseasesMicroRNA in disease regulationCancer-related molecular mechanisms research
Downregulation of hsa_circ_0004543 Activates oxLDL-Induced Vascular Endothelial Cell Proliferation and Angiogenesis | Litcius