Comprehensive Risk Assessment of Carbon Nanotubes Used for Agricultural Applications
Sajedeh Rezaei Cherati, Muhammad Anas, Shijie Liu, Sudha Shanmugam, Kamal Pandey, Steven Angtuaco, Randal S. Shelton, Aida N. Khalfaoui, Savenka V. Alena, Erin B. Porter, Todd Fite, Huaixuan Cao, Micah J. Green, Alexei G. Basnakian, Mariya V. Khodakovskaya
Abstract
Carbon-based nanomaterials (CBNs) are often used for potential agricultural applications. Since CBNs applied to plants can easily enter plant organs and reach the human diet, the consequences of the introduction of CBNs into the food chain need to be investigated. We created a platform for a comprehensive investigation of the possible health risks of multiwalled carbon nanotubes (CNTs) accumulated in the organs of exposed tomato plants. Quantification and visualization of CNTs absorbed by plant organs were determined by microwave-induced heating (MIH) and radio frequency (RF) heating methods. Feeding mice with CNT-contaminated tomatoes showed an absence of toxicity for all assessed animal organs. The amount of CNTs accumulated inside the organs of mice fed with CNT-containing fruits was assessed by an RF heating technique and was found to be negligible. Our work provides the experimental evidence that the amount of CNTs accumulated in plant organs as a result of nanofertilization is not sufficient to induce toxicity in mice.