Synthesis, characterization, and antioxidant activity in vitro of selenium-Euryale ferox Salisb. polysaccharide
Fang Dong, Hu‐Zhe Zheng, Woo‐Sik Jeong, Shin‐Kyo Chung, Zhongyuan Qu, Xiang Zou, Chen Liu, Qiong Xiang, Feng Feng
Abstract
Abstract In order to obtain and explore selenide composed of selenium and polysaccharide, three parameters were selected to optimize the synthesis process of selenium- Euryale ferox Salisb. polysaccharide (Se-ESPS) by Box-Behnken design. Furthermore, ESPS-B1 separated from ESPS was selenitized to Se-ESPS-B1 by the optimal synthesis process, then the characterization, and antioxidant activity in vitro of Se-ESPS-B1 were explored. The result manifested that the Se content of Se-ESPS was (2.915 ± 0.03) mg/g according to the optimal synthesis process of Se-ESPS (reaction time at 5 h, reaction temperature at 81 ℃, weight ratio of Na 2 SeO 3 to ESPS at 0.9 g/g). A series of detection results indicated that the characterizations of Se-ESPS-B1 were apparently distinguished from that of ESPS-B1. Moreover, the antioxidant experiments in vitro demonstrated that Se-ESPS-B1 could exert antioxidant activity by scavenging DPPH, ABTS,·OH, and increasing reduction ability. In conclusion, the synthesis process is an effective approach to harvest selenium-polysaccharide, and Se-ESPS-B1 may be utilized as a potential antioxidant or selenium nutritional supplement.