Binding of AP Endonuclease-1 to G-Quadruplex DNA Depends on the N-Terminal Domain, Mg <sup>2+</sup> , and Ionic Strength
Aaron M. Fleming, Shereen A. Howpay Manage, Cynthia J. Burrows
Abstract
promoter dsDNA→G4 folds in solution. Lastly, the addition of the G4 ligand pyridostatin inhibited APE1 binding and cleavage of F-containing G4s but not dsDNA. The biological and medicinal chemistry implications of the results are discussed.
Topics & Concepts
AP siteEndonucleaseDNA-(apurinic or apyrimidinic site) lyaseDNAAP endonucleaseChemistryTranscription (linguistics)Cleavage (geology)BiochemistryBinding siteTranscription factorEnzymeMutantDNA repairMolecular biologyBiophysicsBiologyGeneFracture (geology)PaleontologyPhilosophyLinguisticsDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery